GFP antibody (HRP)

Catalog No. ABIN129721

Supplier Product No.: 600-403-215

Product Details anti-GFP Antibody (HRP)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-403-215

Supplier: Rockland

Purpose: GFP Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.

Characteristics: Synonyms: rabbit anti-GFP antibody peroxidase conjugation, HRP conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Purification: GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Anti-GFP Antibody is designed to detect GFP and its variants. This GFP Peroxidase conjugated antibody has been tested by ELISA and Western blot. This product can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Western Blot Dilution: 1:2,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store GFP Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFP antibody (HRP) (ABIN129721)

Maloverjan, Piirsoo, Kasak, Peil, Østerlund, Kogerman: "Dual function of UNC-51-like kinase 3 (Ulk3) in the Sonic hedgehog signaling pathway." in: The Journal of biological chemistry, Vol. 285, Issue 39, pp. 30079-90, (2010) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Background: Background: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt: P42212