GFP antibody (FITC)

Catalog No. ABIN129717

Supplier Product No.: 600-402-215

Product Details anti-GFP Antibody (FITC)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.: 600-402-215

Supplier: Rockland

Purpose: GFP Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.

Characteristics: Synonyms: rabbit anti-GFP antibody fluorescein conjugation, FITC conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Purification: GFP Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotype: IgG

Labeling Ratio: 7.2

Biological Activity Comment: 2.24

Application Details

Application Notes:

Flow Cytometry Dilution: 1:2000

Application Note: Anti-Green fluorescent protein Fluorescein conjugated Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:10,000-1:25,000

ELISA Dilution: 1:20,000 - 1:40,000

IF Microscopy Dilution: 1:500 - 1:2,500

Other: FACS User optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-GFP antibody (FITC) (ABIN129717)

Daniel, Russ, Guthridge, Raina, Estes, Parsons, Richardson, Schroeder, Zarnescu: "miR-9a mediates the role of Lethal giant larvae as an epithelial growth inhibitor in Drosophila." in: Biology open, Vol. 7, Issue 1, (2018) (PubMed).

Bankoti, Ogawa, Nguyen, Emadi, Couse, Salehi, Fan, Dhall, Wang, Brown, Funari, Tang, Martins: "Differential regulation of Effector and Regulatory T cell function by Blimp1." in: Scientific reports, Vol. 7, Issue 1, pp. 12078, (2017) (PubMed).

Guo, Maeda, Ma, Delgado, Sohn, Miers, Ko, Bannerman, Xu, Wang, Zhou, Takebayashi, Pleasure: "Macroglial plasticity and the origins of reactive astroglia in experimental autoimmune encephalomyelitis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 33, pp. 11914-28, (2011) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Background: Background: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt: P42212