GPX4 antibody (C-Term)

Catalog No. ABIN129715

Supplier Product No.: 600-401-972

Product Details anti-GPX4 Antibody

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Binding Specificity: C-Term

Reactivity: Mouse, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 600-401-972

Supplier: Rockland

Purpose: Glutathione Peroxidase 4 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse GPx4 protein.

Characteristics: Synonyms: rabbit anti-Gpx4 antibody, rabbit anti-glutathione peroxidase 4/Gpx4 antibody, rabbit anti-glutathione peroxidase 4 antibody, PHGPx antibody, Phospholipid hydroperoxide glutathione peroxidase antibody, Gpx-4, Gpx 4

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 19 kDa in size corresponding to GPx4 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:7,000 - 1:30,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.15 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: Gpx4 (GPX4 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Glutathione Peroxidase 4 (GPx4, also known as PHGPx) functions as an antioxidant and also plays a role in redox regulation, sexual maturation, inflammation, apoptosis, and differentiation.  GPx4 was found to have an important role in preventing lipid peroxidation, and has been discussed primarily as the glutathione peroxidase protecting biomembranes against oxidative stress.  GPx4 can react with free hydrogen peroxide as well as with a wide range of lipid hydroperoxides, including those derived from cholesterol and cholesteryl esters.  GPx4 is the only GPx to use phospholipid hydroperoxides as substrates.  It can also reduce thymine hydroperoxide. In contrast to GPx1 and GPx3, GPx4 can use a wide range of reducing substrates in addition to glutathione.  GPx4 is expressed in high levels in testes, suggesting a role in male fertility.  It has also been identified as an enzymatically inactive structural protein of the mitochondrial capsule of sperm.

Gene ID: 625249, 13508533

UniProt: O70325