Cyclin E2 antibody (C-Term)

Catalog No. ABIN129714

Supplier Product No.: 600-401-971

Product Details anti-Cyclin E2 Antibody

Target: Cyclin E2 (CCNE2)

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-971

Supplier: Rockland

Purpose: Cyclin E2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Cyclin E2 protein.

Characteristics: Synonyms: rabbit anti-Cyclin E2 antibody, G1/S-specific cyclin-E2, CCNE2

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 50 kDa in size corresponding to Cyclin E2 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:20,000 - 1:85,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.17 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Cyclin E2

Target: Cyclin E2 (CCNE2)

Alternative Name: Ccne2 (CCNE2 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cyclin E was first identified by its ability to rescue growth of yeast deficient in G1 Cyclins, indicating a role in G1 or G1/S transitions.  Over-expression of Cyclin E has been observed in a variety of human tumors. Multiple isoforms of Cyclin E are expressed in tumors but not in normal tissues, suggesting a post-transcriptional regulation of Cyclin E.  Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27Kip1 and p21Cip1.  The catalytic activity associated with Cyclin E2 complexes is cell cycle regulated and peaks at the G1/S transition.  Unlike Cyclin E1, which is expressed in most proliferating normal and tumor cells, Cyclin E2 levels were low to undetectable in non-transformed cells and increased significantly in tumor-derived cells.

Gene ID: 12448, 3769612

UniProt: Q9Z238

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases