DAXX antibody (AA 250-275)

Catalog No. ABIN129710

Supplier Product No.: 600-401-966

Product Details anti-DAXX Antibody

Target: DAXX (Death-Domain Associated Protein (DAXX))

Binding Specificity: AA 250-275

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAXX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-966

Supplier: Rockland

Purpose: DAXX Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human DAXX protein.

Characteristics: Synonyms: rabbit anti-DAXX Antibody, BING2 antibody, BING-2, DAP 6 antibody, DAP6 antibody, Death associated protein 6 antibody, Death domain associated protein 6 antibody, Fas death domain-associated protein, ETS1-associated protein 1, EAP1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 250-275 of human DAXX protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to DAXX protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2000

ELISA Dilution: 1:7,000 -  1:45,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DAXX

Target: DAXX (Death-Domain Associated Protein (DAXX))

Alternative Name: DAXX (DAXX Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). DAXX, the Death domain-associated protein 6, resides in multiple locations in the nucleus and in the cytoplasm and interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, DAXX functions as a potent transcription corepressor that binds to several sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli through interactions with MCSR1 and PML. These interactions may regulate TNFRSF6-dependent apoptosis through the meditations of the activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. DAXX acts as histone chaperone that facilitates deposition of histone H3.3. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes, the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Anti-DAXX Antibody is useful for researchers interested in Apoptosis, TGF-Beta, MAPK Signaling Pathway, Cancer, Immunology and Nuclear Signaling research.

Gene ID: 1616, 4503257

UniProt: Q9UER7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway