Aubergine antibody (Internal Region)
Rockland 600-401-947
aub
Reactivity: Drosophila melanogaster
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- Aubergine (aub)
- Binding Specificity
- Internal Region
- Reactivity
- Drosophila melanogaster
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 600-401-947
- Supplier
- Rockland
- Purpose
- Aubergine/Sting Antibody
- Cross-Reactivity (Details)
- Anti-Aubergine affinity-purified antibody is directed against Drosophila Aubergine protein.
- Characteristics
- Synonyms: rabbit anti-Aubergine/Sting Antibody, rabbit anti-Aubergine Antibody, rabbit anti-Sting Antibody, Aubergine isoform A
- Purification
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- Sterility
- Sterile filtered
- Immunogen
-
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Drosophila melanogaster Aubergine/Sting protein.
Immunogen Type: Conjugated Peptide
- Isotype
- IgG
-
- Application Notes
-
Application Note: Aubergine affinity-purified antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 98 kDa in size corresponding to Aubergine protein is expected in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000 - 1:10,000
ELISA Dilution: 1:20,000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- Aubergine (aub)
- Alternative Name
- aub
- Target Type
- Species
- Background
- Background: Aubergine (Sting) is a Drosophila protein involved in germ-line development and RNA interference. Drosophila melanogaster has a robust and efficient innate immune system, which reacts to infections ranging from bacteria to fungi and, as discovered recently, viruses as well. The known Drosophila immune responses rely on humoral and cellular activities, similar to those found in the innate immune system of other animals. Recently, RNAi or 'RNA silencing' has arisen as a possible means by which Drosophila can react to specific pathogens, transposons and retroviral elements, in a fashion similar to that of a traditional mammalian adaptive immune system instead of in a more generalized and genome encoded innate immune-based response. RNAi is a highly conserved regulation and defense mechanism, which suppresses gene expression via targeted RNA degradation directed by either exogenous dsRNA (cleaved into siRNAs) or endogenous miRNAs. In plants, RNAi has been found to act as an antiviral immune response system. In Drosophila multiple core RNAi pathway genes have been reported, including piwi, vasa intronic gene (vig), aubergine (aub), armitage (armi), Rm62, r2d2 and Argonaute2 (AGO2).
- Gene ID
- 34524, 17136494
- UniProt
- O76922
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