MYL12A antibody (AA 10-35)

Catalog No. ABIN129691

Supplier Product No.: 600-401-938

Product Details anti-MYL12A Antibody

Target: MYL12A (Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A))

Binding Specificity: AA 10-35

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL12A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-938

Supplier: Rockland

Purpose: Myosin Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein.

Characteristics: Synonyms: rabbit anti-Myosin antibody, Myosin regulatory light polypeptide 12A, Myosin regulatory light chain 12A, Myosin regulatory light chain MRLC3

Purification: affinity purified antibody

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids aa 10-35 of human myosin light chain protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity-purified antibody was tested by ELISA and immunoblotting and was found to be reactive with both the unphosphorylated and mono-phosphorylated forms of the protein.   Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:70,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.71 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-MYL12A antibody (ABIN129691)

Deng, Bhaidani, Sutherland, MacDonald, Walsh et al.: "Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth ..." in: PLoS ONE, Vol. 14, Issue 12, pp. e0226406, (2020) (PubMed).

Lahey, Ronaghan, Shang, Dion, Désilets, Leduc, MacNaughton: "Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180259, (2017) (PubMed).

Logue, Cartagena-Rivera, Baird, Davidson, Chadwick, Waterman: "Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration." in: eLife, Vol. 4, pp. e08314, (2016) (PubMed).

Target Details for MYL12A

Target: MYL12A (Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A))

Alternative Name: MYL12A (MYL12A Products)

Background: Background: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.

Gene ID: 10627

NCBI Accession: NP_001289978

UniProt: J3QRS3