Myosin antibody (AA 12-27)

Catalog No. ABIN129691

Product Details anti-Myosin Antibody

Target: Myosin

Binding Specificity: AA 12-27

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myosin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 12-27 of human myosin light chain protein.

Isotype: IgG

Application Details

Application Notes: This affinity-purified antibody was tested by ELISA and immunoblotting and was found to be reactive with both the unphosphorylated and mono-phosphorylated forms of the protein.   Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.71 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Myosin antibody (ABIN129691)

Deng, Bhaidani, Sutherland, MacDonald, Walsh et al.: "Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth ..." in: PLoS ONE, Vol. 14, Issue 12, pp. e0226406, (2020) (PubMed).

Lahey, Ronaghan, Shang, Dion, Désilets, Leduc, MacNaughton: "Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180259, (2017) (PubMed).

Logue, Cartagena-Rivera, Baird, Davidson, Chadwick, Waterman: "Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration." in: eLife, Vol. 4, pp. e08314, (2016) (PubMed).

Target Details for Myosin

Target: Myosin

Abstract: Myosin Products

Synonyms: CG3201 antibody, DmMLC_C antibody, Dmel\\CG3201 antibody, MyoII antibody, mlc-c antibody, nmELC antibody, MYOSIN antibody, myosin 1 antibody, DFNA4 antibody, DFNA4A antibody, MHC16 antibody, MYH17 antibody, NMHC II-C antibody, NMHC-II-C antibody, PNMHH antibody, myosin antibody, MYH1 antibody, MYH2 antibody, MYH3 antibody, MYH4 antibody, MYH8 antibody, adult antibody, embryonic antibody, 2400004E04Rik antibody, II-C antibody, NHMCII antibody, DROMHC antibody, Dm nmII antibody, DmnmII antibody, Dronm-MII antibody, E(br) antibody, MHC antibody, Mhc-c antibody, Mhc-c[1] antibody, Myo antibody, Myo II antibody, NMM antibody, Zip antibody, anon-WO0140519.37 antibody, l(2)02957 antibody, nmMHC antibody, nmy-2 antibody, zpr antibody, DmelCG15792 antibody, CG15792 antibody, Myosin light chain cytoplasmic antibody, hypothetical protein antibody, myosin 1 antibody, myosin heavy chain 9 antibody, myosin heavy chain 14 antibody, myosin, heavy chain 1B, skeletal muscle antibody, myosin, heavy polypeptide 14 antibody, zipper antibody, Mlc-c antibody, T22C1.6 antibody, ATM1 antibody, MYH9 antibody, MYH14 antibody, Myh14 antibody, MYH1B antibody, zip antibody

Background: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.  This sequence differs from that of the sarcomeric/
Synonyms: Myosin regulatory light polypeptide 9 Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 Myosin regulatory light chain 2, smooth muscle isoform Myosin RLC MLC-2C 20 kDa myosin light chain

Gene ID: 98932, 38605043

UniProt: P24844