ECT2 antibody (pThr790)

Catalog No. ABIN129688

Supplier Product No.: 600-401-935

Product Details anti-ECT2 Antibody

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Binding Specificity: pThr790

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-935

Supplier: Rockland

Purpose: ECT2 phospho T790 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against the phosphorylated form of human ECT2 protein at the pT790 residue.

Characteristics: Synonyms: rabbit anti-ECT2 pT790 Antibody, ECT 2 antibody, ECT-2 protein antibody, Epithelial cell transforming sequence 2 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kDa in size by western blotting in the appropriate cell lysate or extract.  Less than 2 % reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is believed to be phospho specific for ECT2 phosphorylated at the pT790 residue.  Experiments to generate more conclusive evidence are currently under preparation.

Western Blot Dilution: 1:1,000 - 1:4,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:10,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ECT2

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Alternative Name: ECT2 (ECT2 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42.  ECT2 may be phosphorylated during G₂ and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division.  Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.

Gene ID: 1894, 21735572

UniProt: Q9H8V3

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization