Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Cross-Reactivity
Mouse (Murine), Rat (Rattus), Chimpanzee, Chicken, Sheep (Ovine)
Characteristics
Concentration Definition: by UV absorbance at 280 nm
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 114-125 of human eIF3f protein.
EIF3F
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size by western blotting in the appropriate cell lysate or extract.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
You, Dooley, Kim, Kim, Xu, Goodman, Hornberger: "A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling." in: Science signaling, Vol. 11, Issue 530, (2018) (PubMed).
Ham, Caldow, Chhen, Chee, Wang, Proud, Lynch, Koopman: "Glycine restores the anabolic response to leucine in a mouse model of acute inflammation." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 11, pp. E970-81, (2017) (PubMed).
Wada, Kato, Okutsu, Miyaki, Suzuki, Yan, Schiaffino, Asahara, Ushida, Akimoto: "Translational suppression of atrophic regulators by miR-23a integrates resistance to skeletal muscle atrophy." in: The Journal of biological chemistry, (2011) (PubMed).
Target
EIF3F
(Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))
EIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits. Synonyms: eIF 3 epsilon antibody, eIF3 p47 antibody, eIF3 p47 subunit antibody, EIF3S5 antibody, Eukaryotic translation initiation factor 3 subunit 5 antibody