EIF3F antibody (AA 100-125)

Catalog No. ABIN129687

Product Details anti-EIF3F Antibody

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Binding Specificity: AA 100-125

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: EIF3F Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human eIF3f protein.

Characteristics: Synonyms: rabbit anti-eIF3F Antibody, eIF-3 epsilon antibody, eIF3 p47 antibody, eIF3-p47 subunit antibody, EIF3S5 antibody, Eukaryotic translation initiation factor 3 subunit 5 antibody, Eukaryotic translation initiation factor 3 subunit F, Deubiquitinating enzyme eIF3f

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human eIF3f protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:10,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-EIF3F antibody (ABIN129687)

You, Dooley, Kim, Kim, Xu, Goodman, Hornberger: "A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling." in: Science signaling, Vol. 11, Issue 530, (2018) (PubMed).

Ham, Caldow, Chhen, Chee, Wang, Proud, Lynch, Koopman: "Glycine restores the anabolic response to leucine in a mouse model of acute inflammation." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 11, pp. E970-81, (2017) (PubMed).

Wada, Kato, Okutsu, Miyaki, Suzuki, Yan, Schiaffino, Asahara, Ushida, Akimoto: "Translational suppression of atrophic regulators by miR-23a integrates resistance to skeletal muscle atrophy." in: The Journal of biological chemistry, (2011) (PubMed).

Target Details for EIF3F

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Alternative Name: EIF3F (EIF3F Products)

Background: Background: eIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.

Gene ID: 8665, 6685511

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization