MCM2 antibody (AA 15-40)

Catalog No. ABIN129678

Supplier Product No.: 600-401-922

Product Details anti-MCM2 Antibody

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Binding Specificity: AA 15-40

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-922

Supplier: Rockland

Purpose: MCM2 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human MCM2 protein.

Characteristics: Synonyms: rabbit anti-MCM2 antibody, MCM-2, BM28, CCNL1, CDCL1, Minichromosome maintenance complex component 2, Nuclear protein BM28, DNA replication licensing factor MCM2

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal region near amino acids 15-40 of human MCM2 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 100 kDa in size corresponding to phosphorylated or unphosphorylated MCM2 proteins by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2 (MCM2 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. MCM2, also called DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog or Nuclear Protein BM28) is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by protein kinases CDC2 and CDC7.  Double serines at positions 26 and 27 are conserved in rat and mouse.  High homology >88 % to similar proteins from several other species.  Expected broad reactivity to various lysates.

Gene ID: 4171, 33356547

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA