FBXO9 antibody (AA 425-447)

Catalog No. ABIN129674

Supplier Product No.: 600-401-918

Product Details anti-FBXO9 Antibody

Target: FBXO9 (F-Box Protein 9 (FBXO9))

Binding Specificity: AA 425-447

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-918

Supplier: Rockland

Purpose: F-Box Only Protein 9 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human FBOX9 protein.

Characteristics: Synonyms: rabbit anti-F-Box Only Protein 9 antibody, rabbit anti-FBXO9 antibody, FBX9, VCIA1, Cross-immune reaction antigen 1 antibody, Renal carcinoma antigen NY-REN-57 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-447 of human FBOX9 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user.  Although the predicted MW of FBOX9 isoform 1 is 52 kDa, antibody reactivity against MCF7 whole cell lysates shows a predominant band at 100 kDa.  This band is believed to be FBOX9 and the higher apparent MW may be due to its association with other proteins.  Isoforms 1, 2 and 3 have reported molecular weights of 52.3, 51.1 and 47.3 kDa, respectively.

Western Blot Dilution: 1:1000

ELISA Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FBXO9

Target: FBXO9 (F-Box Protein 9 (FBXO9))

Alternative Name: FBXO9 (FBXO9 Products)

Background: Background: F-box only protein 9 (also called FBOX9 and Fbp9) is a member of the F-box protein family, which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Anti-F-Box 9 Antibody is useful for researchers interested in Multiple Myeloma and Epilepsy, as well as the immune system and Ubiquitin pathway research.

Gene ID: 26268, 53692184

UniProt: Q9UK97