Target

HIS2AV

Binding Specificity

pSer137

Reactivity

Drosophila melanogaster

Host

Rabbit

Compatible Secondaries

Clonality

Polyclonal

Application

ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Purpose

Histone H2AvD phosphoS137 Antibody

Cross-Reactivity (Details)

Affinity purified Anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue.

Characteristics

Synonyms: rabbit anti-H2AvD pS137 antibody, rabbit anti-Histone H2A.v pS137 antibody, H2AvD protein antibody, H2A.F/Z, H2A.Z, H2AvD, His2AvD, His2Av

Purification

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility

Sterile filtered

Immunogen

Immunogen: Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein.

Immunogen Type: Conjugated Peptide

Isotype

IgG

Application Notes

Immunohistochemistry Dilution: 2 μg/mL

Application Note: Histone H2AvD pS137 Antibody is tested in ELISA, Immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein.

Western Blot Dilution: 1:400 - 1:1,600

ELISA Dilution: 1:29,000 - 1:30,000

Other: User Optimized

Restrictions

For Research Use only

Format

Liquid

Concentration

1.0 mg/ml

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date

12 months

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Target: HIS2AV
Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.
Gene ID: 43229, 17738227
UniProt: P08985

HIS2AV (pSer137) antibody

Product Details

Application Details

Handling

References

Target Details

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