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NOXO1 antibody (AA 238-252)

Rockland 600-401-899 NOXO1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN129665
Supplier Product No.: 600-401-899
  • Target See all NOXO1 Antibodies
    NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))
    Binding Specificity
    • 7
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 238-252
    Reactivity
    • 21
    • 1
    • 1
    • 1
    Human
    Host
    • 19
    • 1
    • 1
    Rabbit
    Clonality
    • 21
    Polyclonal
    Conjugate
    • 13
    • 2
    • 2
    • 2
    • 1
    • 1
    This NOXO1 antibody is un-conjugated
    Application
    • 16
    • 13
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-401-899
    Supplier
    Rockland
    Purpose
    NOXO1 Antibody
    Cross-Reactivity (Details)
    Anti-NOXO-1 antibody is directed against human NOXO1 protein.
    Characteristics
    Synonyms: rabbit anti-NOXO1 antibody, NOXO-1, NOXO 1, NADPH oxidase organizer 1 antibody, NADPH oxidase regulatory protein antibody, Nox organizer 1 antibody, Nox organizing protein 1 antibody, Regulatory protein P41NOX antibody, SH3 and PX domain-containing protein 5, P41NOX, SH3PXD5
    Purification
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: NOXO1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 238-252 of human NOXO1 protein.

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
    Top Product
    Discover our top product NOXO1 Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 5-10 μg/mL

    Application Note: Anti-NOXO1 antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:1,000 - 1:10,000

    ELISA Dilution: 1:20,000 - 1:100,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.17 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))
    Alternative Name
    NOXO1 (NOXO1 Products)
    Background
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells.  NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region.  NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme.
    Gene ID
    124056, 16198473
    UniProt
    Q8NFA2
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