MARK2 antibody (pThr595)

Catalog No. ABIN129661

Product Details anti-MARK2 Antibody

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Binding Specificity: pThr595

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Purpose: MARK2 phospho T595 Antibody

Cross-Reactivity (Details): MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue.

Characteristics: Synonyms: rabbit anti-MARK2 pT595 antibody, MARK-2, MARK 2, ELKL motif kinase 1, EMK1, EMK-1, MAP/microtubule affinity regulating kinase 2 antibody, PAR1 homolog antibody, Serine/threonine protein kinase MARK2 antibody, Par-1b, Par1b, PAR1 homolog b

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a.  The immunogen peptide was phosphorylated at the residue corresponding to T595.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-MARK2pT595 antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kDa corresponding to MARK2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:250 - 1:1,000

ELISA Dilution: 1:1,000 - 1:3,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.6 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MARK2

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Alternative Name: MARK2 (MARK2 Products)

Background: Background: MARK2 pT595 antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses.  MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens].  EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer.  Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2.  These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it.  Both forms were found to be co-expressed in a number of selected cell lines and tissue samples.  Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed.  This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein.   Multiple splice variants exist for this protein.

Gene ID: 2011

UniProt: Q7KZI7

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection