MARK2 antibody (Isoform A, pThr595)

Catalog No. ABIN129661

Product Details anti-MARK2 Antibody

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Binding Specificity: Isoform A, pThr595

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARK2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Cross-Reactivity: Dog (Canine), Sheep (Ovine), Mouse (Murine), Rat (Rattus)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 . The immunogen peptide was phosphorylated at the residue corresponding to T595.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kDa corresponding to MARK2 by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.6 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for MARK2

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Alternative Name: MARK2 (MARK2 Products)

Synonyms: EMK-1 antibody, EMK1 antibody, PAR-1 antibody, Par-1b antibody, Par1b antibody, AU024026 antibody, Emk antibody, Par-1 antibody, mKIAA4207 antibody, microtubule affinity regulating kinase 2 antibody, MAP/microtubule affinity regulating kinase 2 antibody, MARK2 antibody, Mark2 antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses.  MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens].  EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer.  Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2.  These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it.  Both forms were found to be co-expressed in a number of selected cell lines and tissue samples.  Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed.  This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein.   Multiple splice variants exist for this protein.
Synonyms: ELKL motif kinase antibody, EMK1 antibody, MAP/microtubule affinity regulating kinase 2 antibody, PAR1 homolog antibody, Serine/threonine protein kinase MARK2 antibod

Gene ID: 2011

UniProt: Q7KZI7, Q7KZ17

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection