beta Actin antibody (AA 350-375)

Catalog No. ABIN129657

Supplier Product No.: 600-401-886

Product Details anti-beta Actin Antibody

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Binding Specificity: AA 350-375

Reactivity: Human, Mouse, Leopard Frog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-886

Supplier: Rockland

Purpose: Beta Actin Antibody

Cross-Reactivity (Details): beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse.

Characteristics: Synonyms: rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Sterility: Sterile filtered

Immunogen:

Immunogen: beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Product Specific Information:

What can the beta-Actin antibody ABIN129657 be used for? This polyclonal, unconjugated Rabbit beta-Actin Antibody is suitable for the detection of beta-Actin (ACTB) by immunohistochemistry, ELISA and Western blotting. The antibody recognizes human beta-actin.

What validation data is available for this beta-Actin antibody? The antibody has been used in 31 publications to date, which are listed below and can be viewed at Pubmed. There are 10 product images available showing the performance of the beta-Actin antibody in western blots. Use our high-quality antibody to reliably detect beta-Actin.

What is the function of Actin? Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. (UniProt)

Application Details

Application Notes:

Application Note: Anti-beta Actin Antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:4,000

ELISA Dilution: 1:10,000 - 1:40,000

IF Microscopy Dilution: 1:500 - 1:2,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store beta-Actin Loading Control Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-beta Actin antibody (ABIN129657)

Olive, Qin, DeCristo, Laszewski, Greathouse, McAllister: "Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies." in: PLoS ONE, Vol. 13, Issue 6, pp. e0198790, (2019) (PubMed).

Dai, Luftig: "Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 200, Issue 5, pp. 1727-1736, (2019) (PubMed).

Borroni, Emanuelli, Shah, Ilić, Apel-Sarid, Paolini, Manikoth Ayyathan, Koganti, Levy-Cohen, Blank: "Smurf2 regulates stability and the autophagic-lysosomal turnover of lamin A and its disease-associated form progerin." in: Aging cell, Vol. 17, Issue 2, (2018) (PubMed).

Coleman, Ha, Haeryfar, Barr, Kim: "Cathepsin B plays a key role in optimal production of the influenza A virus." in: Journal of virology & antiviral research, Vol. 2018, pp. 1-20, (2018) (PubMed).

Lodola, Laforenza, Cattaneo, Ruffinatti, Poletto, Massa, Tancredi, Zuccolo, Khdar, Riccardi, Biggiogera, Rosti, Guerra, Moccia: "VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells." in: Oncotarget, Vol. 8, Issue 56, pp. 95223-95246, (2017) (PubMed).

Chen, Wang, Man, Li, Shan: "Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice." in: Oncology letters, Vol. 13, Issue 4, pp. 2207-2215, (2017) (PubMed).

Rae, Hocum, Bii, Deeg, Trobridge: "A novel retroviral mutagenesis screen identifies prognostic genes in RUNX1 mediated myeloid leukemogenesis." in: Oncotarget, Vol. 6, Issue 31, pp. 30664-74, (2016) (PubMed).

Koh, Powell, Blake, Hoffman, Hopkins, Feng: "Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel." in: Oncology reports, Vol. 34, Issue 3, pp. 1589-98, (2016) (PubMed).

Campos, Qiu, Gomero, Wakefield, Horner, Brutkowski, Han, Solecki, Frase, Bongiovanni, dAzzo: "Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier." in: Nature communications, Vol. 7, pp. 11876, (2016) (PubMed).

Hopkins, Feng, Liu, Parker, Koh: "Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells." in: International journal of oncology, Vol. 46, Issue 5, pp. 2267-76, (2015) (PubMed).

Maragkoudaki, Vaiopoulou, Theodoropoulos, Legaki, Sechi, Karamanolis, Zografos, Gazouli: "Specific detection of OCT4 isoforms in inflammatory bowel disease." in: Gut pathogens, Vol. 7, pp. 25, (2015) (PubMed).

Zha, Xia, Ding, Choi, Yang, Dong, Yan, Huang, Ding: "MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression." in: Cell death & disease, Vol. 5, pp. e1417, (2015) (PubMed).

Nelson, Estus, Abner, Parikh, Malik, Neltner, Ighodaro, Wang, Wilfred, Wang, Kukull, Nandakumar, Farman, Poon, Corrada, Kawas, Cribbs, Bennett, Schneider, Larson, Crane, Valladares, Schmitt, Kryscio et al.: "ABCC9 gene polymorphism is associated with hippocampal sclerosis of aging pathology. ..." in: Acta neuropathologica, Vol. 127, Issue 6, pp. 825-43, (2015) (PubMed).

Wang, Choi, Ding, Yang, Ngoka, Lee, Zha, Mao, Jin, Ren, Cowell, Huang, Shi, Cui, Ding: "HOXC9 directly regulates distinct sets of genes to coordinate diverse cellular processes during neuronal differentiation." in: BMC genomics, Vol. 14, pp. 830, (2014) (PubMed).

Dragoni, Laforenza, Bonetti, Reforgiato, Poletto, Lodola, Bottino, Guido, Rappa, Pareek, Tomasello, Guarrera, Cinelli, Aronica, Guerra, Barosi, Tanzi, Rosti, Moccia: "Enhanced expression of Stim, Orai, and TRPC transcripts and proteins in endothelial progenitor cells isolated from patients with primary myelofibrosis." in: PLoS ONE, Vol. 9, Issue 3, pp. e91099, (2014) (PubMed).

Dragoni, Turin, Laforenza, Potenza, Bottino, Glasnov, Prestia, Ferulli, Saitta, Mosca, Guerra, Rosti, Luinetti, Ganini, Porta, Pedrazzoli, Tanzi, Montagna, Moccia: "Store-operated Ca2+ entry does not control proliferation in primary cultures of human metastatic renal cellular carcinoma." in: BioMed research international, Vol. 2014, pp. 739494, (2014) (PubMed).

Wang, Danaher, Miller, Berger, Nubia, Wilfred, Neltner, Norris, Nelson: "Expression of miR-15/107 family microRNAs in human tissues and cultured rat brain cells." in: Genomics, proteomics & bioinformatics, Vol. 12, Issue 1, pp. 19-30, (2014) (PubMed).

Papaconstantinou, Zeglinas, Gazouli, Nastos, Yiallourou, Lykoudis, Evangelou, Papalois, Papaioannou, Vlachogiannakos, Tzathas: "Effect of infliximab on the healing of intestinal anastomosis. An experimental study in rats." in: International journal of surgery (London, England), Vol. 12, Issue 9, pp. 969-75, (2014) (PubMed).

Laforenza, Scaffino, Gastaldi: "Aquaporin-10 represents an alternative pathway for glycerol efflux from human adipocytes." in: PLoS ONE, Vol. 8, Issue 1, pp. e54474, (2013) (PubMed).

Lodola, Laforenza, Bonetti, Lim, Dragoni, Bottino, Ong, Guerra, Ganini, Massa, Manzoni, Ambudkar, Genazzani, Rosti, Pedrazzoli, Tanzi, Moccia, Porta: "Store-operated Ca2+ entry is remodelled and controls in vitro angiogenesis in endothelial progenitor cells isolated from tumoral patients." in: PLoS ONE, Vol. 7, Issue 9, pp. e42541, (2013) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB (ACTB Products)

Background: Background: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction