SLIT3 antibody (AA 1155-1180)

Catalog No. ABIN129655

Supplier Product No.: 600-401-884

Product Details anti-SLIT3 Antibody

Target: SLIT3 (Slit Homolog 3 (SLIT3))

Binding Specificity: AA 1155-1180

Reactivity: Mouse, Human, Rat, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-884

Supplier: Rockland

Purpose: SLIT3 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human SLIT-3 protein.

Characteristics: Synonyms: rabbit anti-SLIT-3 antibody, SLIT3, SLIT 3, Slit homolog 3 protein, SLIL2, Multiple epidermal growth factor-like domains 5 antibody, Multiple EGF-like domains protein 5 antibody, MEGF 5 antibody, MEGF5 antibody, KIAA0814 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1155-1180 of Human SLIT-3 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 168 kDa corresponding to full length protein as well as multiple bands corresponding to isoforms of SLIT-3 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SLIT3

Target: SLIT3 (Slit Homolog 3 (SLIT3))

Alternative Name: SLIT3 (SLIT3 Products)

Background: Background: SLIT-3 (also known as multiple epidermal growth factor-like domain 5 and Slit homolog 3 protein) is a Slit protein.  The 'slit' gene has been shown to play a critical role in central nervous system midline formation. In addition to SLIT3 there are two additional human 'slit' homologs, which are termed SLIT1 and SLIT2.  Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein.  SLIT proteins may also participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.  Slit-3 is a secreted protein predominantly expressed in thyroid.  Multiple isoforms have been reported for this product.

Gene ID: 6586, 11321571

UniProt: O75094

Pathways: Regulation of Cell Size