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TUBA1B antibody (AA 425-451)

Rockland 600-401-880 TUBA1B Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, FM Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN129653
Supplier Product No.: 600-401-880
  • Target See all TUBA1B Antibodies
    TUBA1B (Tubulin, alpha 1B (TUBA1B))
    Binding Specificity
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 425-451
    Reactivity
    • 37
    • 14
    • 12
    • 5
    • 5
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 27
    • 13
    • 1
    Rabbit
    Clonality
    • 28
    • 13
    Polyclonal
    Conjugate
    • 24
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TUBA1B antibody is un-conjugated
    Application
    • 30
    • 21
    • 16
    • 13
    • 10
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)
    Supplier Product No.
    600-401-880
    Supplier
    Rockland
    Purpose
    Alpha-Tubulin Antibody
    Cross-Reactivity (Details)
    Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.
    Characteristics
    Synonyms: rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-tubulin antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
    Purification
    The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
    Top Product
    Discover our top product TUBA1B Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 1:500 - 1:2,000

    Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

    Western Blot Dilution: 1:1,000 - 1:5,000

    ELISA Dilution: 1:90,000

    IF Microscopy Dilution: 1:500 - 1:2,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store Anti-Tubulin Loading Control Antibody at -20° C prior to opening. Aliquot Loading Control Antibody contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
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    Ghosh, Abdelwahab, Reeber, Reidel, Marklew, Garrison, Lee, Dang, Herring, Glish, Kesimer, Tarran: "Little Cigars are More Toxic than Cigarettes and Uniquely Change the Airway Gene and Protein Expression." in: Scientific reports, Vol. 7, pp. 46239, (2018) (PubMed).

    Kapadia, Bijelić, Zhao, Ma, Stojanovich, Milošević, Andjus, Šakić: "Effects of sustained i.c.v. infusion of lupus CSF and autoantibodies on behavioral phenotype and neuronal calcium signaling." in: Acta neuropathologica communications, Vol. 5, Issue 1, pp. 70, (2018) (PubMed).

    Liao, Ren, Peterson, Marshak, Yau, Gamlin, Dacey: "Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations." in: The Journal of comparative neurology, Vol. 524, Issue 14, pp. 2845-72, (2017) (PubMed).

    Dilworth, Upadhyay, Bonnafous, Edoo, Bourbigot, Pesek-Jardim, Gudavicius, Serpa, Petrotchenko, Borchers, Nelson, Mackereth: "The basic tilted helix bundle domain of the prolyl isomerase FKBP25 is a novel double-stranded RNA binding module." in: Nucleic acids research, Vol. 45, Issue 20, pp. 11989-12004, (2017) (PubMed).

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  • Target
    TUBA1B (Tubulin, alpha 1B (TUBA1B))
    Alternative Name
    TUBA1B (TUBA1B Products)
    Background
    Background: Tubulin Loading Control Antibody recognizes microtubules which are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control. Anti-Alpha-tubulin antibody is ideal for investigators involved in cell cycle protein research.
    Gene ID
    17986283
    UniProt
    P68363
    Pathways
    Microtubule Dynamics, M Phase
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