APC1 antibody (pSer377)
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- Target See all APC1 (ANAPC1) Antibodies
- APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))
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Binding Specificity
- pSer377
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This APC1 antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 600-401-877
- Supplier
- Rockland
- Purpose
- APC1 phospho S377 Antibody
- Cross-Reactivity (Details)
- This antibody is specific for phosphorylated human APC1 protein at the pS377 residue.
- Characteristics
- Synonyms: rabbit anti-APC1 pS377 Antibody, APC-1, APC 1, ANAPC1 antibody, Anaphase promoting complex subunit 1 antibody, Cyclosome subunit 1 antibody, MCPR antibody, Meiotic checkpoint regulator antibody
- Purification
- This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pS377 of Human Apc1 protein.
Immunogen Type: Conjugated Peptide
- Isotype
- IgG
- Top Product
- Discover our top product ANAPC1 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 5.0 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 215 kDa in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200 - 1:1,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.21 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))
- Alternative Name
- ANAPC1 (ANAPC1 Products)
- Background
- Background: APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.
- Gene ID
- 64682, 12056971
- UniProt
- Q9H1A4
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