APC1 antibody (pSer377)

Catalog No. ABIN129650

Supplier Product No.: 600-401-877

The Rabbit Polyclonal anti-APC1 antibody has been validated for ELISA, IHC and WB. It is suitable to detect APC1 in samples from Human.

Quick Overview for APC1 antibody (pSer377) (ABIN129650)

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-APC1 Antibody

Binding Specificity: pSer377

Supplier Product No.: 600-401-877

Supplier: Rockland

Purpose: APC1 phospho S377 Antibody

Cross-Reactivity (Details): This antibody is specific for phosphorylated human APC1 protein at the pS377 residue.

Characteristics: Synonyms: rabbit anti-APC1 pS377 Antibody, APC-1, APC 1, ANAPC1 antibody, Anaphase promoting complex subunit 1 antibody, Cyclosome subunit 1 antibody, MCPR antibody, Meiotic checkpoint regulator antibody

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pS377 of Human Apc1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 5.0 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 215 kDa in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:1,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.21 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for APC1

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Alternative Name: ANAPC1

Background: Background: APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.

Gene ID: 64682, 12056971

UniProt: Q9H1A4