RIF1 antibody (AA 2375-2419)

Catalog No. ABIN129646

Supplier Product No.: 600-401-871

Product Details anti-RIF1 Antibody

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Binding Specificity: AA 2375-2419

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-871

Supplier: Rockland

Purpose: Rif1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Rif1 protein.

Characteristics: Synonyms: rabbit anti-Rif1 antibody, Rif-1, Rif 1, Telomere-associated protein RIF1, Rap1 interacting factor 1 antibody, Rap1 interacting factor 1 homolog antibody, mRif1, Rap-1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 2375-2419 of Mouse Rif1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 265 kDa in size corresponding to Rif1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.6 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-RIF1 antibody (ABIN129646)

Tanaka, Muto, Shima, Katoh, Sax, Tajima, Brydun, Ikura, Yoshizawa, Masai, Hoshikawa, Noda, Nio, Ochiai, Igarashi: "Epigenetic Regulation of the Blimp-1 Gene (Prdm1) in B Cells Involves Bach2 and Histone Deacetylase 3." in: The Journal of biological chemistry, Vol. 291, Issue 12, pp. 6316-30, (2016) (PubMed).

Target Details for RIF1

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Alternative Name: Rif1 (RIF1 Products)

Background: Background: Rif1 (also known as telomere-associated protein RIF1 and Rap1-interacting factor 1) is a mouse ortholog of the yeast Rif1 family of telomere-associated proteins identified on the basis of its high expression in primordial germ cells and embryo-derived pluripotent stem cell lines.  Mouse Rif1 is a protein involved in negative regulation of telomere length.  It is also localized to ATM dependent DNA damage foci and may act at the chromatin level to allow access to some factors.

Gene ID: 51869, 68565756

UniProt: Q6PR54

Pathways: Stem Cell Maintenance