SLIT1 antibody (AA 485-515)

Catalog No. ABIN129640

Product Details anti-SLIT1 Antibody

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Binding Specificity: AA 485-515

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Slit1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse SLIT-1 protein.

Characteristics: Synonyms: rabbit anti-Slit-1 antibody, Slit1, Slit 1, Slit homolog 1 protein

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 485-515 of mouse SLIT-1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect multiple bands corresponding to cleavage products of SLIT-1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SLIT1

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Alternative Name: Slit1 (SLIT1 Products)

Background: Background: SLIT-1 (also known as KIAA0813, MEGF4, multiple epidermal growth factor-like domains 4 and Slit homolog 1 protein) is a Slit protein.  This protein is a ligand for the Roundabout (Robo) receptors and acts as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb.  Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. In situ hybridization studies indicated that the rat SLIT-1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. This data suggests that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.  Alternative splicing isoforms have been identified for Slit1 protein.

Gene ID: 20562, 256355216

UniProt: Q80TR4

Pathways: Regulation of Cell Size