DLL1 antibody (AA 150-175)

Catalog No. ABIN129638

Supplier Product No.: 600-401-858

Product Details anti-DLL1 Antibody

Target: DLL1 (delta-Like 1 (DLL1))

Binding Specificity: AA 150-175

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-858

Supplier: Rockland

Purpose: DELTA-1 Antibody

Cross-Reactivity (Details): The antibody detects DELTA-1 from mouse.

Characteristics: Synonyms: rabbit anti-Delta1 antibody, Delta-1, Delta 1, Delta-like protein 1 antibody, Drosophila Delta homolog 1, Delta-like protein 1, Dll1-soluble form, Dll1-EC, Dll1-derived cell-associated form, Dll1-TMIC, Dll1

Purification: Anti-DELTA-1 Antibody was affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 150-175 of Mouse DELTA-1.  Swiss Prot accession Q61483. 

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2 μg/mL

Application Note: Anti-DELTA-1 Antibody has been tested for use in ELISA against the immunizing peptide, western blot, and in IHC.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:100 - 1:1,000

ELISA Dilution: 1:3,000 - 1:12,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-DLL1 antibody (ABIN129638)

Meliou, Kerezoudis, Tosios, Kiaris: "Notch 1 Receptor, Delta 1 Ligand and HES 1 Transcription Factor are Expressed in the Lining Epithelium of Periapical Cysts (Preliminary Study)." in: The open dentistry journal, Vol. 4, pp. 153-8, (2011) (PubMed).

Zhang, Zeng, Zhang, Jiao, Li, Jing, Ouyang, Yuan, Chai, Che, Zhang, Yang: "Suppressor of cytokine signaling 3 promotes bone marrow cells to differentiate into CD8+ T lymphocytes in lung tissue via up-regulating Notch1 expression." in: Cancer research, Vol. 69, Issue 4, pp. 1578-86, (2009) (PubMed).

Target Details for DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Alternative Name: Dll1 (DLL1 Products)

Background: Background: Delta-like protein 1 is a protein that in humans is encoded by the DLL1 gene. The Notch signaling pathway has pleiotropic functions during mammalian embryogenesis. It is required for the patterning and differentiation of the presomitic and somitic paraxial mesoderm and of the neural tube. DELTA-1 is a mouse homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. Anti-Delta-like protein 1 antibody would be suitable for researchers in Cell Biology and Signal Transduction.

Gene ID: 13388

NCBI Accession: NP_031891

UniProt: Q61483

Pathways: Notch Signaling, Stem Cell Maintenance