AJUBA antibody (AA 224-239)

Catalog No. ABIN129634

Product Details anti-AJUBA Antibody

Target: AJUBA (Ajuba LIM Protein (AJUBA))

Binding Specificity: AA 224-239

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AJUBA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: AJUBA Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Ajuba.

Characteristics: Synonyms: rabbit anti-Ajuba Antibody, Ajuba, JUB protein, ajuba homolog isoform 1, LIM domain-containing protein ajuba

Purification: The product was affinity purified from antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 224-239 of Human Ajuba.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size corresponding to AJUBA by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:20,000 - 1:80,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.67 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for AJUBA

Target: AJUBA (Ajuba LIM Protein (AJUBA))

Alternative Name: AJUBA (AJUBA Products)

Background: Background: Human AJUBA (also called JUB protein and ajuba homolog isoform 1) is a LIM domain protein suggested to bind and regulate the activity of Aurora A.  Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.

Gene ID: 84962, 14249622

UniProt: Q96IF1

Pathways: Chromatin Binding, Cell-Cell Junction Organization