DIAPH2 antibody (C-Term)

Catalog No. ABIN129631

Supplier Product No.: 600-401-851

Product Details anti-DIAPH2 Antibody

Target: DIAPH2 (Diaphanous Homolog 2 (DIAPH2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIAPH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-851

Supplier: Rockland

Purpose: DIA-2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human DIA-2.

Characteristics: Synonyms: rabbit anti-DIA-2 Antibody, DIA 2, DIA drome, DIA2, DIAPH2, Diaphanous 2, Diaphanous related formin 2, Diaphorase 2, DRF2, POF, POF2

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kDa in size corresponding to DIA-2 by western blotting in the appropriate cell lysate or extract.   Alternate splice variants have been described for this protein.  This antibody will only react with DIA-156 (isoform 1 of DIA-2).

Western Blot Dilution: 1:1,000 - 1:6,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:12,000 - 1:60,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.4 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DIAPH2

Target: DIAPH2 (Diaphanous Homolog 2 (DIAPH2))

Alternative Name: DIAPH2 (DIAPH2 Products)

Background: Background: DIA-2 (also called DIA drome, Dia2, DIAPH2, Diaphanous 2, Diaphanous related formin 2, Diaphorase 2, DRF2, POF and POF2) may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described. DIA-2 is expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes and can be found from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.   Isoform 1 is referred to as DIA-156, whereas isoform 2 is called DIA-12C.

Gene ID: 1730, 5803003

UniProt: O60879