ZIC2 antibody (Internal Region)

Catalog No. ABIN129629

Product Details anti-ZIC2 Antibody

Target: ZIC2

Binding Specificity: AA 180-210, Internal Region

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZIC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: ZIC-2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse ZIC2 protein.

Characteristics: Synonyms: rabbit anti-ZIC-2 antibody, ZIC 2, ZIC2, zinc finger protein of the cerebellum 2, Zinc finger protein ZIC 2, HPE5, Zic family member 2 and odd-paired Drosophila homolog

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 180-210 of Mouse ZIC-2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to ZIC2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ZIC2

Target: ZIC2

Alternative Name: Zic2 (ZIC2 Products)

Background: Background: ZIC2 (also known as zinc finger protein of the cerebellum 2, HPE5, Zic family member 2 and odd-paired Drosophila homolog) is a nuclear protein that functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. In mouse, knockout of Zic1 leads to aplasia of cerebellum and skeletal abnormalities. Mutation in Zic2 and Zic3 in mouse and/or human cause holoprosencephaly and heterotaxis, respectively. Holoprosencephaly is the most common structural anomaly of the human brain.

UniProt: Q62520

Pathways: Tube Formation