SLIT2 antibody (AA 484-500)

Catalog No. ABIN129628

Product Details anti-SLIT2 Antibody

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Binding Specificity: AA 484-500

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Chicken

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 484-500 of Human SLIT-2 protein.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 165 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  For western blotting block the membrane with goat serum.  Do not block with non-fat dry milk.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for SLIT2

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Alternative Name: SLIT-2 (SLIT2 Products)

Synonyms: SLIL3 antibody, Slit-2 antibody, Drad-1 antibody, E030015M03Rik antibody, E130320P19Rik antibody, Slil3 antibody, mKIAA4141 antibody, Slit antibody, slil3 antibody, slit-2 antibody, slit2-a antibody, SLIT2 antibody, slit guidance ligand 2 antibody, slit homolog 2 (Drosophila) antibody, slit guidance ligand 2 S homeolog antibody, SLIT2 antibody, Slit2 antibody, slit2 antibody, slit2.S antibody

Background: SLIT-2 is thought to act as a molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homologue receptors.  SLIT-1 and SLIT-2 seem to be essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development SLIT-2 may play a role in guiding commissural axons once they reach the floor plate by modulating the response to netrin.  In vitro, SLIT-2 silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex.  In vitro, only commissural axons that crossed the midline responded to SLIT-2.  In the developing visual system this protein functions as a repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm.  SLIT-2 is a secreted protein predominantly expressed in fetal lung and kidney, and adult spinal cord.  Weak expression is observed in adult adrenal gland, thyroid, trachea and other tissues examined.  Multiple isoforms have been reported for this product.
Synonyms: Drad-1 antibody, E030015M03Rik antibody, E130320P19Rik antibody, FLJ14420 antibody, Slil 3 antibody, Slil3 antibody

Gene ID: 9353, 4759146

UniProt: O94813

Pathways: Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration