GLI2 antibody (AA 30-65)

Catalog No. ABIN129627

Supplier Product No.: 600-401-845

Product Details anti-GLI2 Antibody

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Binding Specificity: AA 30-65

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-845

Supplier: Rockland

Purpose: GLI2 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human Gli-2 protein.

Characteristics: Synonyms: rabbit anti-GLI-2 antibody, GLI2, zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein antibody, THP antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human Gli-2 (isoform a).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot.  Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively).  Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:30,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GLI2 antibody (ABIN129627)

Gonnissen, Isebaert, Perneel, McKee, Van Utterbeeck, Lerut, Verrill, Bryant, Joniau, Muschel, Haustermans: "Patched 1 Expression Correlates with Biochemical Relapse in High-Risk Prostate Cancer Patients." in: The American journal of pathology, Vol. 188, Issue 3, pp. 795-804, (2019) (PubMed).

Gonnissen, Isebaert, McKee, Dok, Haustermans, Muschel: "The hedgehog inhibitor GANT61 sensitizes prostate cancer cells to ionizing radiation both in vitro and in vivo." in: Oncotarget, Vol. 7, Issue 51, pp. 84286-84298, (2018) (PubMed).

Gonnissen, Isebaert, Perneel, McKee, Verrill, Bryant, Van Utterbeeck, Lerut, Haustermans, Muschel: "Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer." in: BMC cancer, Vol. 17, Issue 1, pp. 634, (2018) (PubMed).

Gonnissen, Isebaert, McKee, Muschel, Haustermans: "The Effect of Metformin and GANT61 Combinations on the Radiosensitivity of Prostate Cancer Cells." in: International journal of molecular sciences, Vol. 18, Issue 2, (2017) (PubMed).

Roessler, Ermilov, Grange, Wang, Grachtchouk, Dlugosz, Muenke: "A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2." in: Human molecular genetics, Vol. 14, Issue 15, pp. 2181-8, (2005) (PubMed).

Target Details for GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Alternative Name: GLI2 (GLI2 Products)

Background: Background: Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila.

Gene ID: 2736

NCBI Accession: NP_005261

UniProt: P10070

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis