DLL4 antibody (Internal Region)

Catalog No. ABIN129625

Supplier Product No.: 600-401-696

Product Details anti-DLL4 Antibody

Target: DLL4 (delta-Like 4 (DLL4))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-696

Supplier: Rockland

Purpose: DELTA-4 Antibody

Cross-Reactivity (Details): DELTA-4 affinity purified antibody is directed against human DELTA-4.

Characteristics: Synonyms: rabbit anti-DELTA4 antibody, DELTA-4, delta 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor, DELTA 4

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 10 μg/mL to 20 μg/mL

Application Note: Anti-DELTA-4 has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 74 kDa in size corresponding to DELTA-4 by western blotting in the appropriate cell lysate or extract. For best results use Goat Serum as blocking agent in western blotting.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.88 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-DLL4 antibody (ABIN129625)

Wu, Esumi, Watanabe, Chen, Nakamura, Nakamura, Kometani, Minato, Yanagawa, Akashi, Sakimura, Kaneko, Tamamaki: "Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon." in: Development (Cambridge, England), Vol. 138, Issue 12, pp. 2499-509, (2011) (PubMed).

Zhang, Zeng, Zhang, Jiao, Li, Jing, Ouyang, Yuan, Chai, Che, Zhang, Yang: "Suppressor of cytokine signaling 3 promotes bone marrow cells to differentiate into CD8+ T lymphocytes in lung tissue via up-regulating Notch1 expression." in: Cancer research, Vol. 69, Issue 4, pp. 1578-86, (2009) (PubMed).

Indraccolo, Minuzzo, Masiero, Pusceddu, Persano, Moserle, Reboldi, Favaro, Mecarozzi, Di Mario, Screpanti, Ponzoni, Doglioni, Amadori: "Cross-talk between tumor and endothelial cells involving the Notch3-Dll4 interaction marks escape from tumor dormancy." in: Cancer research, Vol. 69, Issue 4, pp. 1314-23, (2009) (PubMed).

Lauret, Catelain, Titeux, Poirault, Dando, Dorsch, Villeval, Groseil, Vainchenker, Sainteny, Bennaceur-Griscelli: "Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential." in: Leukemia, Vol. 18, Issue 4, pp. 788-97, (2004) (PubMed).

Tohda, Murata-Ohsawa, Sakano, Nara: "Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines." in: International journal of oncology, Vol. 22, Issue 5, pp. 1073-9, (2003) (PubMed).

Liu, Shirakawa, Li, Soma, Oka, Dotto, Fairman, Velazquez, Herlyn: "Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis." in: Molecular and cellular biology, Vol. 23, Issue 1, pp. 14-25, (2002) (PubMed).

Target Details for DLL4

Target: DLL4 (delta-Like 4 (DLL4))

Alternative Name: DLL4 (DLL4 Products)

Background: Background: DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.  DELTA-4  plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.

Gene ID: 54567, 9506545

UniProt: Q9NR61

Pathways: Notch Signaling