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GLI3 antibody (AA 30-60)

Rockland 600-401-694 GLI3 Reactivity: Human WB, ELISA, IHC, IF, FM, MA Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN129623
Supplier Product No.: 600-401-694
  • Target See all GLI3 Antibodies
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Binding Specificity
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 30-60
    Reactivity
    • 35
    • 15
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 29
    • 5
    • 2
    Rabbit
    Clonality
    • 24
    • 12
    Polyclonal
    Conjugate
    • 22
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This GLI3 antibody is un-conjugated
    Application
    • 16
    • 10
    • 7
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)
    Supplier Product No.
    600-401-694
    Supplier
    Rockland
    Purpose
    GLI3 Antibody
    Cross-Reactivity (Details)
    This affinity-purified antibody is directed against human Gli-3 protein.
    Characteristics
    Synonyms: Rabbit anti-GLI-3 antibody, Transcriptional activator GLI3, Gli 3, GLI3 form of 190 kDa, GLI3 form of 83 kDa
    Purification
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein.

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
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    Discover our top product GLI3 Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 0.5 mg/mL - 5 μg/mL

    Application Note: This antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:6,000 - 1:30,000

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Hehemann, Kalmanek, Choe, Dynda, Hu, Quek, Harrington, Stupp, McVary, Podlasek: "Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence." in: Neurourology and urodynamics, Vol. 37, Issue 8, pp. 2551-2559, (2019) (PubMed).

  • Target
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Alternative Name
    GLI3 (GLI3 Products)
    Background
    Background: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and cranio-facial development.  Two isoforms of human Gli-3 have been reported.  One is the full-length protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.
    Gene ID
    2737, 119393899
    UniProt
    P10071
    Pathways
    Hedgehog Signaling
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