EGR1 antibody (AA 85-115)

Catalog No. ABIN129622

Product Details anti-EGR1 Antibody

Target: EGR1 (Early Growth Response 1 (EGR1))

Binding Specificity: AA 85-115

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: EGR-1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human EGR-1 protein.

Characteristics: Synonyms: rabbit anti-EGR-1 Antibody, EGR1, EGR 1, AT225 antibody, Early growth response 1 antibody, KROX24 antibody, Nerve growth factor-induced protein A antibody, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, ZNF225, Zinc finger protein 225, Zinc finger protein Krox-24

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 85-115 of Human EGR-1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2 μg/mL to 20 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~58 kDa in size corresponding to EGR-1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:4,000 - 1:16,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.93 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-EGR1 antibody (ABIN129622)

Weaver, Hellstrom, Brown, Andrews, Dymov, Diorio, Zhang, Szyf, Meaney: "The methylated-DNA binding protein MBD2 enhances NGFI-A (egr-1)-mediated transcriptional activation of the glucocorticoid receptor." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 369, Issue 1652, (2015) (PubMed).

Hellstrom, Dhir, Diorio, Meaney: "Maternal licking regulates hippocampal glucocorticoid receptor transcription through a thyroid hormone-serotonin-NGFI-A signalling cascade." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 367, Issue 1601, pp. 2495-510, (2012) (PubMed).

Target Details for EGR1

Target: EGR1 (Early Growth Response 1 (EGR1))

Alternative Name: EGR1 (EGR1 Products)

Background: Background: EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site).  EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation.   EGR-1 is a nuclear protein induced by growth factors. Expression has been identified in a variety of cancers.

Gene ID: 1958

NCBI Accession: NP_001955

UniProt: P18146

Pathways: Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity