ROBO1 antibody (AA 1625-1650)

Catalog No. ABIN129621

Supplier Product No.: 600-401-692

Product Details anti-ROBO1 Antibody

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Binding Specificity: AA 1625-1650

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROBO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-692

Supplier: Rockland

Purpose: ROBO-1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human ROBO-1 protein.

Characteristics: Synonyms: rabbit anti-ROBO1 antibody, ROBO 1, ROBO-1, hROBO-1, Roundabout homolog 1, Deleted in U twenty twenty, DUTT1, DUTT-1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2 μg/mL to 10 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, western blot, and immunohistochemistry. It may be suitable for immunofluorescence and IP.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~181 kDa in size corresponding to ROBO-1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:30,000 - 1:60,000

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ROBO1 antibody (ABIN129621)

Li, Wang, Chen, Liang: "[Auditory neuropathy in children(analysis of 14 cases)]." in: Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology, Vol. 19, Issue 1, pp. 19-21, (2006) (PubMed).

Target Details for ROBO1

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Alternative Name: ROBO1 (ROBO1 Products)

Background: Background: ROBO-1 (also called Roundabout homolog 1 precursor and Deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2.  The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex.  ROBO-1 may also be required for lung development. ROBO-1 is a type I membrane protein.  ROBO-1 is a widely expressed protein with the exception of the kidney.  Defects in ROBO1 may be a cause of breast and lung cancer. ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. Multiple splice variants have been identified for this protein.

Gene ID: 6091

UniProt: Q2M1J3

Pathways: Positive Regulation of Endopeptidase Activity