E2F1 antibody (pSer364)

Catalog No. ABIN129619

Supplier Product No.: 600-401-690

Product Details anti-E2F1 Antibody

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Binding Specificity: pSer364

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-690

Supplier: Rockland

Purpose: E2F-1 phospho S364 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against the phosphorylated form of human E2F-1 at the pS364 residue.

Characteristics: Synonyms: rabbit anti-E2F-1 pS364 Antibody, E2F 1 antibody, transcription factor E2F1 antibody, E2F1 antibody, E2F transcription factor 1 antibody, PBR 3 antibody, PBR3 antibody, Retinoblastoma binding protein 3, RBBP-3, pRB-binding protein E2F-1, Retinoblastoma-associated protein 1, RBAP-1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human E2F-1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2 mg/mL - 20 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to phosphorylated E2F-1 by western blotting in the appropriate cell lysate or extract.  Less than 0.5 % reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS364 of E2F-1.

Western Blot Dilution: 1:250 - 1:2,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.80 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1 (E2F1 Products)

Background: Background: E2F-1 (also known as transcription factor E2F-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'.  The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains conserved through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain, a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and two other members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis as well as it can block adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Anti-E2F-1 pS364 Antibody is useful for researchers involved in transcription factor and DNA binding research.

Gene ID: 1869, 12669911

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy