SETDB1 antibody (AA 1058-1075)

Catalog No. ABIN129618

Product Details anti-SETDB1 Antibody

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Binding Specificity: AA 1058-1075

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1058-1075 of human ESET.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Western blotting shows reactivity specific for human ESET detecting a band at approximately 170 kDa.  Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane.  Reactivity in other immunoassays is unknown.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.17 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-SETDB1 antibody (ABIN129618)

Sarraf, Stancheva: "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly." in: Molecular cell, Vol. 15, Issue 4, pp. 595-605, (2004) (PubMed).

Wang, An, Cao, Xia, Erdjument-Bromage, Chatton, Tempst, Roeder, Zhang: "mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression." in: Molecular cell, Vol. 12, Issue 2, pp. 475-87, (2003) (PubMed).

Schultz, Ayyanathan, Negorev, Maul, Rauscher: "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins." in: Genes & development, Vol. 16, Issue 8, pp. 919-32, (2002) (PubMed).

Harte, Wu, Carrasquillo, Matera: "Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids." in: Cytogenetics and cell genetics, Vol. 84, Issue 1-2, pp. 83-6, (1999) (PubMed).

Target Details for SETDB1

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Alternative Name: ESET (SETDB1 Products)

Synonyms: ESET antibody, H3-K9-HMTase4 antibody, KG1T antibody, KMT1E antibody, TDRD21 antibody, AU022152 antibody, mKIAA0067 antibody, SETDB1 antibody, NV10069 antibody, fc07d03 antibody, si:ch211-81a5.6 antibody, wu:fc07d03 antibody, im:7143091 antibody, wu:fa04a12 antibody, wu:fj80h02 antibody, zgc:152899 antibody, SET domain bifurcated 1 antibody, SET domain, bifurcated 1 antibody, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 11 antibody, histone-lysine N-methyltransferase SETDB1 antibody, SET domain, bifurcated 1a antibody, SET domain bifurcated 1 L homeolog antibody, SET domain, bifurcated 1b antibody, SETDB1 antibody, Setdb1 antibody, MLLT11 antibody, LOC100120014 antibody, LOC100461671 antibody, setdb1a antibody, setdb1.L antibody, setdb1b antibody

Background: The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3.  H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.
Synonyms: Histone lysine N methyltransferase antibody, Histone-lysine N-methyltransferase SETDB1 antibody, KG1T antibody, KIAA00067 antibody, KIAA0067 antibody, KMT1E antibody, Lysine N-methyltransferase 1E antibody

Gene ID: 9869, 224177469

UniProt: Q15047