ATAD5 antibody (AA 63-76)

Catalog No. ABIN129617

Supplier Product No.: 600-401-688

Product Details anti-ATAD5 Antibody

Target: ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))

Binding Specificity: AA 63-76

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATAD5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 600-401-688

Supplier: Rockland

Purpose: ELG-1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Elg1 protein.

Characteristics: Synonyms: rabbit anti-ELG-1 antibody, ELG1, ELG 1, FRAG1, ATP (GTP)-binding protein, ATPase Family AAA Domain-Containing Protein 5, Chromosome fragility associated gene 1 protein, ATAD5, C17orf41

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Elg1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:3,000 - 1:15,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.50 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ATAD5

Target: ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))

Alternative Name: ATAD5 (ATAD5 Products)

Background: Background: ELG1 (also known as ATP(GTP)-binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120 kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207 kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.

Gene ID: 79915, 21615526

UniProt: Q96QE3