RAD54L antibody (AA 1-25)

Catalog No. ABIN129616

Supplier Product No.: 600-401-684

Product Details anti-RAD54L Antibody

Target: RAD54L (RAD54-Like (RAD54L))

Binding Specificity: AA 1-25

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD54L antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-684

Supplier: Rockland

Purpose: RAD54 Antibody

Cross-Reactivity (Details): BLAST analysis indicates 100 % homology of the immunizing sequence with RAD54 from human.

Characteristics: Synonyms: rabbit anti-RAD54 antibody, RAD-54, RAD 54, RAD54A, RAD-54A, RAD 54A, RAD54L, RAD-54L, RAD 54L, DNA repair and recombination protein RAD54-like antibody, hHR 54 antibody, hHR54 antibody, hRAD 54 antibody, hRAD54 antibody

Purification: This product is an affinity-purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near aa 1-25 of Human RAD54 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 84 kDa in size corresponding to RAD54 protein by western blotting in the appropriate cell lysate or extract.  Splice variants exist for this protein that may result in the detection of lower molecular weight bands.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RAD54L

Target: RAD54L (RAD54-Like (RAD54L))

Alternative Name: RAD54L (RAD54L Products)

Background: Background: RAD54, also known as hHR54, HR54, hRAD54 and RAD54A, belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination.

Gene ID: 603761, 216548186

UniProt: Q92698

Pathways: DNA Damage Repair