RAD52 antibody (AA 350-375)

Catalog No. ABIN129613

Product Details anti-RAD52 Antibody

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Binding Specificity: AA 350-375

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD52 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: RAD52 Antibody

Cross-Reactivity (Details): Reactivity occurs against human RAD52 protein.

Characteristics: Synonyms: rabbit anti-RAD52 antibody, RAD-52, RAD 52, DNA repair protein RAD52 homolog antibody, RAD 52 antibody, RAD52 homolog antibody, recombination protein RAD52 antibody

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 350-375 of the Human Rad 52 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested by WB and ELISA. Anti-RAD52 is useful in western blotting using HeLa nuclear extracts.  Dilutions for western blotting represent a starting point dilution and further optimization may be required.  The antibody detects a band of approximately 63.0 kDa (predicted molecular weight: 46.3 kDa). Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RAD52

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Alternative Name: RAD52 (RAD52 Products)

Background: Background: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair.   Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene.  The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).

Gene ID: 5893

NCBI Accession: NP_602296

UniProt: P43351

Pathways: DNA Damage Repair