Aurora Kinase B antibody (Internal Region)

Catalog No. ABIN129612

Supplier Product No.: 600-401-677

Product Details anti-Aurora Kinase B Antibody

Target: Aurora Kinase B (AURKB)

Binding Specificity: Internal Region

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-677

Supplier: Rockland

Purpose: AURORA KINASE B phospho T232 Antibody

Cross-Reactivity (Details): Anti-Phospho Aurora B pT232 affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue.

Characteristics: Synonyms: rabbit anti-Aurora B pT232 Antibody, Phospho Aurora B, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Phospho pT232 Aurora B antibody has been tested for use in ELISA, immunohistochemistry, and by western blot.  See below for specific protocol.  Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract.  HeLa cell lysate can be used as a positive control.

Western Blot Dilution: 1:250 - 1:2,000

ELISA Dilution: 1:10,000 - 1:30,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.21 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Phospho Specific Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Aurora Kinase B antibody (ABIN129612)

Barroso-Vilares, Macedo, Reis, Warren, Compton, Logarinho: "Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence." in: EMBO reports, Vol. 21, Issue 5, pp. e49248, (2020) (PubMed).

Achuthankutty, Thakur, Haahr, Hoffmann, Drainas, Bizard, Weischenfeldt, Hickson, Mailand: "Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability." in: The Journal of cell biology, Vol. 218, Issue 12, pp. 3943-3953, (2020) (PubMed).

Novais-Cruz, Alba Abad, van IJcken, Galjart, Jeyaprakash, Maiato, Ferrás: "Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription." in: eLife, Vol. 7, (2019) (PubMed).

Liu, Kwon, Mannino, Yang, Renda, Khodjakov, Pellman: "Nuclear envelope assembly defects link mitotic errors to chromothripsis." in: Nature, Vol. 561, Issue 7724, pp. 551-555, (2019) (PubMed).

Xie, Lin, Wu, Tan, Cheng, Zhang: "Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway." in: Oncotarget, Vol. 9, Issue 17, pp. 13783-13795, (2018) (PubMed).

Skowyra, Allan, Saurin, Clarke: "USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability." in: Cell reports, Vol. 23, Issue 3, pp. 852-865, (2018) (PubMed).

Drpic, Almeida, Aguiar, Renda, Damas, Lewin, Larkin, Khodjakov, Maiato: "Chromosome Segregation Is Biased by Kinetochore Size." in: Current biology : CB, Vol. 28, Issue 9, pp. 1344-1356.e5, (2018) (PubMed).

de Cárcer, Venkateswaran, Salgueiro, El Bakkali, Somogyi, Rowald, Montañés, Sanclemente, Escobar, de Martino, McGranahan, Malumbres, Sotillo: "Plk1 overexpression induces chromosomal instability and suppresses tumor development." in: Nature communications, Vol. 9, Issue 1, pp. 3012, (2018) (PubMed).

Shrestha, Conti, Tamura, Braun, Ramalingam, Cieslinski, Ries, Draviam: "Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells." in: Nature communications, Vol. 8, Issue 1, pp. 150, (2017) (PubMed).

Mo, Zhuang, Liu, Yao, Qin, Su, Zang, Wang, Zhang, Dou, Tian, Teng, Niu, Hill, Fang, Ding, Fu, Yao: "Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation." in: Nature chemical biology, Vol. 12, Issue 4, pp. 226-32, (2016) (PubMed).

Redli, Gasic, Meraldi, Nigg, Santamaria: "The Ska complex promotes Aurora B activity to ensure chromosome biorientation." in: The Journal of cell biology, Vol. 215, Issue 1, pp. 77-93, (2016) (PubMed).

Asghar, Lajeunesse, Dulla, Combes, Thebault, Nigg, Elowe: "Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation." in: Nature communications, Vol. 6, pp. 8364, (2015) (PubMed).

Ohishi, Muramatsu, Yoshida, Seimiya: "TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation." in: Molecular and cellular biology, Vol. 34, Issue 13, pp. 2464-78, (2014) (PubMed).

Carretero, Ruiz-Torres, Rodríguez-Corsino, Barthelemy, Losada: "Pds5B is required for cohesion establishment and Aurora B accumulation at centromeres." in: The EMBO journal, Vol. 32, Issue 22, pp. 2938-49, (2014) (PubMed).

Nijenhuis, Vallardi, Teixeira, Kops, Saurin: "Negative feedback at kinetochores underlies a responsive spindle checkpoint signal." in: Nature cell biology, Vol. 16, Issue 12, pp. 1257-64, (2014) (PubMed).

Rivera, Ghenoiu, Rodríguez-Corsino, Mochida, Funabiki, Losada: "Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex." in: The EMBO journal, Vol. 31, Issue 6, pp. 1467-79, (2012) (PubMed).

Mackay, Makise, Ullman: "Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint." in: The Journal of cell biology, Vol. 191, Issue 5, pp. 923-31, (2011) (PubMed).

Wheatley, Henzing, Dodson, Khaled, Earnshaw: "Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5655-60, (2004) (PubMed).

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AURKB (AURKB Products)

Background: Background: Aurora Kinase B (Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. Anti-AUROA B pT232 Antibody is useful for researchers interested in gene expression, DNA damage, cytokinesis, and transcription activities.

Gene ID: 9212, 83776600

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades