Concentration Definition: by UV absorbance at 280 nm
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 and WHIP2 with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.
This affinity purified antibody has been tested for use in western blotting against HEK293 whole cell lysates. The antibody is also functional by ELISA. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa). Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.10 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Target
WRNIP1
(Werner Helicase Interacting Protein 1 (WRNIP1))
Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2). Synonyms: ATPase WRNIP 1 antibody, ATPase WRNIP1 antibody, bA420G6.2 antibody, FLJ22526 antibody, Putative helicase RUVBL antibody, RP11 420G6.2 antibody, Werner helicasae interacting protein 1 antibody