WRNIP1 antibody (Internal Region)
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- Target See all WRNIP1 Antibodies
- WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This WRNIP1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 600-401-676
- Supplier
- Rockland
- Purpose
- WHIP Antibody
- Cross-Reactivity (Details)
- Reactivity is expected against human, mouse, rat and monkey WHIP1 protein.
- Characteristics
- Synonyms: rabbit anti-WHIP antibody, ATPase WRNIP 1 antibody, ATPase WRNIP1 antibody, Werner helicasae interacting protein 1 antibody, WRNIP-1
- Purification
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.
Immunogen Type: Conjugated Peptide
- Isotype
- IgG
- Top Product
- Discover our top product WRNIP1 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity purified antibody has been tested by WB and ELISA. Anti-WHIP is useful in western blotting against HEK293 whole cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa). Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:10,000 - 1:40,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.10 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))
- Alternative Name
- WRNIP1 (WRNIP1 Products)
- Background
- Background: Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2).
- Gene ID
- 56897, 55661735
- UniProt
- Q96S55
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