FANCG antibody (AA 1-25)

Catalog No. ABIN129607

Supplier Product No.: 600-401-671

Product Details anti-FANCG Antibody

Target: FANCG (Fanconi Anemia Complementation Group G (FANCG))

Binding Specificity: AA 1-25

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FANCG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-671

Supplier: Rockland

Purpose: FANCG Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human FANCG protein.

Characteristics: Synonyms: rabbit anti-FANCG antibody, FANC-G, FANC G, Fanconi anemia group G protein, DNA repair protein XRCC9 antibody, Fanconi anaemia complementation group G antibody, Protein FACG antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region near amino acids 1-25 of human FANCG protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 69 kDa in size corresponding to FANCG by western blotting in the appropriate human tissue.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.93 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FANCG

Target: FANCG (Fanconi Anemia Complementation Group G (FANCG))

Alternative Name: FANCG (FANCG Products)

Background: Background: FANCG (also called Protein FACG or DNA-repair protein XRCC9) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCG may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. This protein may also function as a tumor suppressor gene. FANCG belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. FANCG is mainly found within the nucleus although some protein is localized in the cytoplasm.  This protein is highly expressed in testis and thymus and is also found in lymphoblasts. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity, they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G.

Gene ID: 2189, 4759336

UniProt: O15287

Pathways: DNA Damage Repair