HUS1B antibody (AA 100-125)

Catalog No. ABIN129605

Supplier Product No.: 600-401-498

Product Details anti-HUS1B Antibody

Target: HUS1B (HUS1 Checkpoint Homolog B (HUS1B))

Binding Specificity: AA 100-125

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUS1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-498

Supplier: Rockland

Purpose: HUS1B Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human Hus1B protein.

Characteristics: Synonyms: rabbit anti-HUS1B antibody, Checkpoint protein HUS1B, hHUS1B, HUS 1B, HUS-1B, HUS1 Checkpoint Clamp Component B, HUS1B, HUS1 (S. Pombe) Checkpoint Homolog B, HUS1 Checkpoint Homolog B (S. Pombe), HUS1 Checkpoint Homolog B

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human Hus1B protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-HUS1B antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to Hus1B protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.75 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HUS1B

Target: HUS1B (HUS1 Checkpoint Homolog B (HUS1B))

Alternative Name: HUS1B (HUS1B Products)

Background: Background: The protein encoded by the Hus1B gene is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the checkpoint protein RAD1 but not with RAD9. Over expression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to HUS1. Hus1B is expressed strongly in testis, less in spleen, thymus, prostate, colon and leukocytes. Anti-HUSB1 Antibody is useful for researchers interested in DNA damage and repair.

Gene ID: 135458, 31077215

UniProt: Q8NHY5