FBXO5 antibody (N-Term)

Catalog No. ABIN129601

Supplier Product No.: 600-401-490

Product Details anti-FBXO5 Antibody

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-490

Supplier: Rockland

Purpose: Fbp5A Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human Fbp5A protein.

Characteristics: Synonyms: rabbit anti-F-Protein Only Protein 5/Fbp5A antibody, rabbit anti-F-Protein Only Protein 5 antibody, rabbit anti-Fbp5A antibody, FBXO5, EMI1, FBX5, F-Box Protein Antibody, Early mitotic inhibitor 1 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human Fbp5A protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:3,000

Application Note: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 51 kDa in size corresponding to Fbp5A protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.3 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Alternative Name: FBXO5 (FBXO5 Products)

Background: Background: Fbp5A is a member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to Xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase-promoting complex.

Gene ID: 26271, 6912366

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases