ATG7 antibody (AA 558-569)

Catalog No. ABIN129600

Product Details anti-ATG7 Antibody

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Binding Specificity: AA 558-569

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 558-569 of Human Apg7 protein.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 78 kDa in size corresponding to human Apg7 by western blotting in the appropriate cell lysate or extract.   Human Apg7 has been reported to form a homodimer at approximately 160 kDa.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.46 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-ATG7 antibody (ABIN129600)

Bhattacharya, Parillon, Zeng, Han, Eissa: "Deficiency of autophagy in dendritic cells protects against experimental autoimmune encephalomyelitis." in: The Journal of biological chemistry, Vol. 289, Issue 38, pp. 26525-32, (2015) (PubMed).

Abdel Fattah, Bhattacharya, Herron, Safdar, Eissa: "Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 11, pp. 5407-16, (2015) (PubMed).

Fung, Chen, Grandis, Duvvuri: "EGFR tyrosine kinase inhibition induces autophagy in cancer cells." in: Cancer biology & therapy, Vol. 13, Issue 14, pp. 1417-24, (2013) (PubMed).

Shin, Fattah, Bhattacharya, Ko, Eissa: "Inflammasome activation by altered proteostasis." in: The Journal of biological chemistry, Vol. 288, Issue 50, pp. 35886-95, (2013) (PubMed).

Liu, Ko, Xu, Fattah, Xiang, Jagannath, Ishii, Komatsu, Eissa: "Transient aggregation of ubiquitinated proteins is a cytosolic unfolded protein response to inflammation and endoplasmic reticulum stress." in: The Journal of biological chemistry, Vol. 287, Issue 23, pp. 19687-98, (2012) (PubMed).

Sarkar, Perlstein, Imarisio, Pineau, Cordenier, Maglathlin, Webster, Lewis, OKane, Schreiber, Rubinsztein: "Small molecules enhance autophagy and reduce toxicity in Huntington's disease models." in: Nature chemical biology, Vol. 3, Issue 6, pp. 331-8, (2007) (PubMed).

Sarkar, Davies, Huang, Tunnacliffe, Rubinsztein: "Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and alpha-synuclein." in: The Journal of biological chemistry, Vol. 282, Issue 8, pp. 5641-52, (2007) (PubMed).

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: Apg7 (ATG7 Products)

Synonyms: APG7-LIKE antibody, APG7L antibody, GSA7 antibody, D8 antibody, UBA1B antibody, UBE1L antibody, UBE2 antibody, Ube1l antibody, 1300004C08Rik antibody, AO090001000744 antibody, atg7 antibody, 1810013K23Rik antibody, Agp7 antibody, Apg7l antibody, Atg7l antibody, ATAPG7 antibody, ATATG7 antibody, ATG7 antibody, AUTOPHAGY 7 antibody, AUTOPHAGY-RELATED 7 antibody, K15I22.10 antibody, K15I22_10 antibody, autophagy related 7 antibody, ubiquitin like modifier activating enzyme 7 antibody, ubiquitin-like modifier activating enzyme 7 antibody, autophagy-related protein 7 antibody, Autophagy-related protein 7 antibody, Apg7p antibody, ThiF family protein antibody, ubiquitin-like conjugating enzyme Atg7 antibody, ATG7 antibody, UBA7 antibody, Uba7 antibody, ANI_1_3368024 antibody, AOR_1_1338164 antibody, PTRG_02236 antibody, PAAG_00287 antibody, PITG_12537 antibody, atg7 antibody, Atg7 antibody, APG7 antibody

Background: Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
Synonyms: APG7 autophagy 7-like (S. cerevisiae) antibody, APG7 like antibody, APG7, S. cerevisiae, homolog of antibody, APG7L antibody, ATG 7 antibody, atg7 antibody, ATG7 autophagy related 7 homolog (S. cerevisiae) antibody

Gene ID: 10533

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy