IBTK antibody (Internal Region)

Catalog No. ABIN129599

Supplier Product No.: 600-401-486

Product Details anti-IBTK Antibody

Target: IBTK (Inhibitor of Bruton Agammaglobulinemia Tyrosine Kinase (IBTK))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IBTK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-486

Supplier: Rockland

Purpose: BTB1 Antibody

Characteristics: Synonyms: rabbit anti-BTB1 Antibody, BTB-1, BTB 1, IBTK, BTKI, Inhibitor of Bruton tyrosine kinase, BTK Binding Protein antibody

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Btb1. Btb1 is a currently uncharacterized human ankyrin repeat protein BAA92655. This protein has been suggested to bind to CUL3.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.63 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IBTK

Target: IBTK (Inhibitor of Bruton Agammaglobulinemia Tyrosine Kinase (IBTK))

Alternative Name: IBTK (IBTK Products)

Background: Background: BTB1 acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. It down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.

Gene ID: 25998

NCBI Accession: NP_001287835

UniProt: Q9P2D0