FBXO43 antibody (N-Term)
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- Target See all FBXO43 Antibodies
- FBXO43 (F-Box Protein 43 (FBXO43))
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Binding Specificity
- N-Term
- Reactivity
- Human, Mouse, Rat, Chimpanzee
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FBXO43 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 600-401-485
- Supplier
- Rockland
- Purpose
- Fbp5B Antibody
- Cross-Reactivity (Details)
- This affinity-purified antibody is directed against human Fbp5B protein.
- Characteristics
- Synonyms: rabbit anti-F-Box Protein 43/Fbp5B antibody, rabbit anti-F-Box Protein 43 antibody, rabbit anti-Fbp5B antibody, F-box only protein 43, Endogenous meiotic inhibitor 2, FBXO43, EMI2
- Purification
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein. Human Fbp5B-2, 466 aa, predicted MW 51.3 kDa
Immunogen Type: Conjugated Peptide
- Isotype
- IgG
- Top Product
- Discover our top product FBXO43 Primary Antibody
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- Application Notes
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Application Note: This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 105 kDa in size corresponding to Fbp5B protein by western blotting in the appropriate cell lysate or extract. Predicted molecular weight is 51 kDa.
Western Blot Dilution: 1:500 - 1:3,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2.21 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- FBXO43 (F-Box Protein 43 (FBXO43))
- Alternative Name
- FBXO43 (FBXO43 Products)
- Background
- Background: Fbp5B (also called F-Box protein 43 or Fbx043) is a relatively new member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class.
- Gene ID
- 286151, 117606351
- UniProt
- Q4G163
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