USP7 antibody (N-Term)

Catalog No. ABIN129596

Supplier Product No.: 600-401-482

Product Details anti-USP7 Antibody

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-482

Supplier: Rockland

Purpose: HAUSP Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human HAUSP protein.

Characteristics: Synonyms: rabbit anti-HAUSP antibody, USP7, Deubiquitinating enzyme 7 antibody, Herpes virus associated ubiquitin specific protease antibody, Ubiquitin carboxyl terminal hydrolase 7 antibody, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human HAUSP protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 130 kDa in size corresponding to HAUSP protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:15,000 - 1:60,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for USP7

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Alternative Name: USP7 (USP7 Products)

Background: Background: HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53, even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.

Gene ID: 7874, 150378533

UniProt: Q93009