SFRP1 antibody

Catalog No. ABIN129592

Supplier Product No.: 600-401-475

Product Details anti-SFRP1 Antibody

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFRP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-475

Supplier: Rockland

Purpose: SFRP1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human SFRP protein.

Characteristics: Synonyms: rabbit anti-SFRP1 antibody, SFRP-1, secreted frizzled related protein 1 antibody, FRP-1 antibody, FRP 1 antibody, FRP1 antibody, FrzA antibody, SARP 2 antibody, SARP2 antibody, Secreted Apoptosis-related Protein 2 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: SFRP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:800

Application Note: This affinity purified antibody has been tested for use in ELISA, western blot, and IHC.  This antibody is suitable for immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Sfrp1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200-1:2,000

ELISA Dilution: 1:5,000 - 1:15,000

IF Microscopy Dilution: Use at a concentration of 5 μg/mL

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-SFRP1 antibody (ABIN129592)

Huang, Zhang, Teng, Lin, Zheng, Yang, Han: "Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma." in: BMC cancer, Vol. 7, pp. 126, (2007) (PubMed).

Garcia-Hoyos, Cantalapiedra, Arroyo, Esteve, Rodríguez, Riveiro, Trujillo, Ramos, Bovolenta, Ayuso: "Evaluation of SFRP1 as a candidate for human retinal dystrophies." in: Molecular vision, Vol. 10, pp. 426-31, (2004) (PubMed).

Han, Amar: "Secreted frizzled-related protein 1 (SFRP1) protects fibroblasts from ceramide-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2832-40, (2004) (PubMed).

Target Details for SFRP1

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Alternative Name: SFRP1 (SFRP1 Products)

Background: Background: Anti-SFRP1 is a Stem Cell Antibody. SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzled-related Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Gene ID: 6422, 56117838

UniProt: Q8N474

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation