Cyclin B1 antibody (pSer126)
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- Target See all Cyclin B1 (CCNB1) Antibodies
- Cyclin B1 (CCNB1)
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Binding Specificity
- AA 120-131, pSer126
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Cyclin B1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Cross-Reactivity
- Rat (Rattus), Dog (Canine), Chimpanzee, Mouse (Murine), Hamster
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 120-131 of Human Cyclin B1 protein.
- Isotype
- IgG
- Top Product
- Discover our top product CCNB1 Primary Antibody
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- Application Notes
- This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2.01 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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Artemis links ATM to G2/M checkpoint recovery via regulation of Cdk1-cyclin B." in: Molecular and cellular biology, Vol. 27, Issue 7, pp. 2625-35, (2007) (PubMed).
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Artemis links ATM to G2/M checkpoint recovery via regulation of Cdk1-cyclin B." in: Molecular and cellular biology, Vol. 27, Issue 7, pp. 2625-35, (2007) (PubMed).
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- Target
- Cyclin B1 (CCNB1)
- Alternative Name
- Cyclin B1 (CCNB1 Products)
- Synonyms
- ccnb antibody, cycb antibody, cb267 antibody, cycb1 antibody, wu:fa19g04 antibody, wu:fb16d01 antibody, wu:fb16e07 antibody, wu:fi21c01 antibody, ccnb1 antibody, MGC53596 antibody, CCNB antibody, Ccnb1-rs1 antibody, Ccnb1-rs13 antibody, CycB1 antibody, Cycb-4 antibody, Cycb-5 antibody, Cycb1-rs1 antibody, cyclin B1 antibody, cyclin B1 S homeolog antibody, ccnb1 antibody, ccnb1.2 antibody, ccnb1.S antibody, CCNB1 antibody, Ccnb1 antibody, ccnb1.2.S antibody
- Background
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Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
Synonyms: Cyclin B1, CCNB1, CCNB, G2/mitotic-specific cyclin-B1 - Gene ID
- 891
- UniProt
- P14635
- Pathways
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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