DNMT3L antibody (AA 150-175)

Catalog No. ABIN129584

Supplier Product No.: 600-401-454

Product Details anti-DNMT3L Antibody

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Binding Specificity: AA 150-175

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Supplier Product No.: 600-401-454

Supplier: Rockland

Purpose: DNMT3L Antibody

Cross-Reactivity (Details): This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76 % identity with mouse.

Characteristics: Synonyms: rabbit anti-DNMT3L antibody, DNA (cytosine-5)-methyltransferase 3-like, DNMT3L, DNMT 3L, DNMT-3L

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-DNMT3L antibody has been tested for use in ELISA against the immunizing peptide.  This antibody has been tested in immunohistochemistry and western blot. This antibody works for western blotting only following immunoprecipitation.  The expected molecular weight of human DNMT3L is 43.6 kDa. Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1000 - 1:3000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.11 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DNMT3L

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Alternative Name: DNMT3L (TRDMT1 Products)

Background: Background: DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.

Gene ID: 29947

NCBI Accession: NP_787063

UniProt: Q9UJW3