PLK1 antibody (pThr210)

Catalog No. ABIN129581

Product Details anti-PLK1 Antibody

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Binding Specificity: AA 205-214, pThr210

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.

Isotype: IgG

Application Details

Application Notes: This affinity-purified antibody has been tested for use in ELISA, IHC and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract.   This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-PLK1 antibody (ABIN129581)

Adler, Müller, Rached, Dekant, Mally: "Modulation of key regulators of mitosis linked to chromosomal instability is an early event in ochratoxin A carcinogenicity." in: Carcinogenesis, Vol. 30, Issue 4, pp. 711-9, (2009) (PubMed).

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: Plk1 (PLK1 Products)

Synonyms: PLK antibody, STPK13 antibody, plk antibody, PLK-1 antibody, Plx1 antibody, stpk13 antibody, Plk antibody, cb525 antibody, cb636 antibody, ik:tdsubc_2d1 antibody, wu:fb37g11 antibody, wu:fb76g03 antibody, xx:tdsubc_2d1 antibody, polo like kinase 1 antibody, polo like kinase 1 S homeolog antibody, polo-like kinase 1 antibody, polo-like kinase 1 (Drosophila) antibody, Serine/threonine-protein kinase plk-1 antibody, PLK1 antibody, plk1.S antibody, plk1 antibody, Plk1 antibody, plk-1 antibody

Background: Polo-like Kinase pT210 Antibody detects phosphorylated Plk1 protein. Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G₁ or S phase and is associated with the phosphorylation of cyclin B₁.  Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression.  Plk-1 has a nuclear localization and accumulates to a maximum during the G₂ and M phases, declines to a nearly undetectable level following mitosis and throughout G₁ phase, and then begins to accumulate again during S phase.  Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.
Synonyms: PLK 1 antibody, PLK antibody, polio like kinase antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody

Gene ID: 5347, 21359873

UniProt: P53350

Pathways: Cell Division Cycle, M Phase