PLK1 antibody (pThr210)

Catalog No. ABIN129581

Product Details anti-PLK1 Antibody

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Binding Specificity: pThr210

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: PLK1 phospho T210 Antibody

Cross-Reactivity (Details): Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein.

Characteristics: Synonyms: rabbit anti-PLK1 pT210 antibody, Serine/threonine-protein kinase PLK1, rabbit anti-polio like kinase 1 antibody, Serine/threonine protein kinase 13 antibody, PLK-1, PLK 1, STPK13

Purification: This antibody was affinity purified.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to an internal region near aa 200-225 of Human Polo-like kinase 1 (Plk1) protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: This affinity-purified antibody has been tested for use in ELISA, IHC, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract.   This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:3,000 - 1:12,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-PLK1 antibody (ABIN129581)

Adler, Müller, Rached, Dekant, Mally: "Modulation of key regulators of mitosis linked to chromosomal instability is an early event in ochratoxin A carcinogenicity." in: Carcinogenesis, Vol. 30, Issue 4, pp. 711-9, (2009) (PubMed).

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1 (PLK1 Products)

Background: Background: Polo-like Kinase pT210 Antibody detects phosphorylated Plk1 protein. Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G₁ or S phase and is associated with the phosphorylation of cyclin B₁.  Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression.  Plk-1 has a nuclear localization and accumulates to a maximum during the G₂ and M phases, declines to a nearly undetectable level following mitosis and throughout G₁ phase, and then begins to accumulate again during S phase.  Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.

Gene ID: 5347, 21359873

UniProt: P53350

Pathways: Cell Division Cycle, M Phase